Western Blot Substrates and Substrate Kits
Thermo Scientific™ NBT Substrate Powder (nitro-blue tetrazolium chloride)
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Novus Biologicals™ UltraScence Femto Plus Western Substrate
Luminol-based enhanced chemiluminescent substrate
Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots
Novus Biologicals™ UltraScence Pico Ultra Western Substrate
Luminol-based enhanced chemiluminescent substrate
MilliporeSigma™ Calbiochem™ TMB, Insoluble
Peroxidase substrate which produces stable, insoluble, aquamarine blue precipitate at reaction site.
Thermo Scientific™ Pierce™ CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Azure Biosystems Radiance Plus
Femtogram HRP substrate for chemiluminescent Western blots (150 mL kit).
Seracare Life Sciences Inc TMB 1-C MEMBRANE HRP SUBSTRATE
Most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. 1-component format of 3, 3', 5, 5' - Tetramethylbenzidine, no reagent preparation, hence, very convenient.
Bio Rad Laboratories Clarity Western ECL Substrate, 500 ml, for Western blotting, HRP compatible, chemiluminescence detection, femtogram sensitivity, 24-hour signal duration
Clarity Western ECL Substrate, 500 ml, for Western blotting, HRP compatible, chemiluminescence detection, femtogram sensitivity, 24-hour signal duration
ProteinSimple Chemiluminescent Substrate
Compatible with Jess, Abby and Wes systems. The chemiluminescent Substrate includes Luminol-S (P/N: 042-233) and Peroxide (P/N: 042-234). Each order contains two bottles of 2.0mL of substrate/bottle.
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST BCIP/NBT
Synonym: BCIP/NBT Alkaline Phosphatase Substrate. Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.
Vector Laboratories TMB Peroxidase (HRP) Substrate Kit, 3,3',5,5'-Tetramethylbenzidine 300 ml
Vector Laboratories TMB Peroxidase (HRP) Substrate Kit, (3,3Æ,5,5Æ-tetramethylbenzidine) produces either a precipitating blue reaction product or a soluble blue reaction product, depending on whether a stabilizer has been added to the solution. This sensitive chromogen can be used for blotting, immunohistochemical, and in situ hybridization applications. TMB stained slides can be dehydrated and permanently mounted. The soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.